My measurements are N = 66 and S = 367 (arbitrary units). Next, measure the signal, S, from the middle of the baseline noise vertically to the top of the peak of interest. First, measure the baseline noise, using the technique discussed in HPLC Solutions #122 to capture the width of the baseline, which is the noise, N, in Figure 1. An example is shown in Figure 1.Įxpand the chromatogram sufficiently to facilitate the measurements, and transfer it to a graphics program or print a copy if it makes the measurements easier. To make this measurement, you’ll need to have a chromatogram under the desired conditions that contains enough baseline to measure the noise accurately – often 3-20 times the width of the peak. Signal-to-noise often is used to help determine the limit of detection or limit of quantification of an HPLC method. One practical application of such measurements is to determine the signal-to-noise ratio (S/N), which we’ll consider here. In HPLC Solutions #122, we looked at how to measure baseline noise from a liquid chromatogram.
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